Localization of 3-nitrotyrosine to rheumatoid and normal synovium
dc.contributor.author | Walsh, David A | |
dc.date.accessioned | 2022-05-12T14:55:25Z | |
dc.date.available | 2022-05-12T14:55:25Z | |
dc.date.issued | 2001-07 | |
dc.identifier.citation | Mapp, P. I. et al. (2001) ‘Localization of 3-Nitrotyrosine to Rheumatoid and Normal Synovium’, ARTHRITIS AND RHEUMATISM -ATLANTA-, 1 January, pp. 1534–1539. | en_US |
dc.identifier.uri | http://hdl.handle.net/20.500.12904/15437 | |
dc.description.abstract | Objective: To determine the localization of 3-nitrotyrosine (3-NT), a footprint marker of peroxynitrite (ONOO-) and other reactive nitrogen species, to the inflamed human synovium and to compare this with normal synovial and nonsynovial tissue of human and animal origin. Methods: Monoclonal and polyclonal antibodies were used to investigate for 3-NT, inducible nitric oxide synthase (iNOS), macrophage marker CD68, and the vascular smooth muscle marker alpha-actin by avidin-biotin immunocytochemistry. Results: In the inflamed synovium, 3-NT was found in the vascular smooth muscle and macrophages. In normal human synovium, 3-NT was present in the vascular smooth muscle and some lining cells and was not associated with immunoreactivity for iNOS. Similarly, 3-NT could be demonstrated in the vascular smooth muscle cells of normal rats and iNOS knockout mice. It was not present in the vascular smooth muscle of healthy, nonsynovial tissue. Conclusion: The synovial vasculature in histologically normal human and naive rodent synovium was alone among the normal tissues studied in exhibiting iNOS-independent immunoreactivity for 3-NT. These findings suggest a physiologic role for ONOO- in normal synovial vascular function. | |
dc.publisher | Arthritis and Rheumatism | en_US |
dc.subject | Nitrotyrosine | en_US |
dc.subject | Rheumatoid | en_US |
dc.subject | Norman synovium | en_US |
dc.subject | Inflammation | en_US |
dc.title | Localization of 3-nitrotyrosine to rheumatoid and normal synovium | en_US |
dc.type | Article | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
rioxxterms.version | NA | en_US |
rioxxterms.versionofrecord | 10.1002/1529-0131(200107)44:7<1534::AID-ART276>3.0.CO;2-# | en_US |
rioxxterms.type | Journal Article/Review | en_US |
refterms.panel | Unspecified | en_US |
html.description.abstract | Objective: To determine the localization of 3-nitrotyrosine (3-NT), a footprint marker of peroxynitrite (ONOO-) and other reactive nitrogen species, to the inflamed human synovium and to compare this with normal synovial and nonsynovial tissue of human and animal origin. Methods: Monoclonal and polyclonal antibodies were used to investigate for 3-NT, inducible nitric oxide synthase (iNOS), macrophage marker CD68, and the vascular smooth muscle marker alpha-actin by avidin-biotin immunocytochemistry. Results: In the inflamed synovium, 3-NT was found in the vascular smooth muscle and macrophages. In normal human synovium, 3-NT was present in the vascular smooth muscle and some lining cells and was not associated with immunoreactivity for iNOS. Similarly, 3-NT could be demonstrated in the vascular smooth muscle cells of normal rats and iNOS knockout mice. It was not present in the vascular smooth muscle of healthy, nonsynovial tissue. Conclusion: The synovial vasculature in histologically normal human and naive rodent synovium was alone among the normal tissues studied in exhibiting iNOS-independent immunoreactivity for 3-NT. These findings suggest a physiologic role for ONOO- in normal synovial vascular function. | en_US |
rioxxterms.funder.project | 94a427429a5bcfef7dd04c33360d80cd | en_US |