Show simple item record

dc.contributor.authorAmbalkar, Shrikant
dc.date.accessioned2020-08-11T15:59:38Z
dc.date.available2020-08-11T15:59:38Z
dc.date.issued2020-05
dc.identifier.citationMonaghan, T. M. et al. (2020) ‘Metagenomics reveals impact of geography and acute diarrheal disease on the Central Indian human gut microbiome’, Gut microbes, pp. 1–24. doi: 10.1080/19490976.2020.1752605.en
dc.identifier.otherPMID: 32459982
dc.identifier.urihttp://hdl.handle.net/20.500.12904/1554
dc.description.abstractBackground: The Central Indian gut microbiome remains grossly understudied. Herein, we sought to investigate the burden of antimicrobial resistance and diarrheal diseases, particularly Clostridioides difficile , in rural-agricultural and urban populations in Central India, where there is widespread unregulated antibiotic use. We utilized shotgun metagenomics to comprehensively characterize the bacterial and viral fractions of the gut microbiome and their encoded functions in 105 participants. Results: We observed distinct rural-urban differences in bacterial and viral populations, with geography exhibiting a greater influence than diarrheal status. Clostridioides difficile disease was more commonly observed in urban subjects, and their microbiomes were enriched in metabolic pathways relating to the metabolism of industrial compounds and genes encoding resistance to 3 rd generation cephalosporins and carbapenems. By linking phages present in the microbiome to their bacterial hosts through CRISPR spacers, phage variation could be directly related to shifts in bacterial populations, with the auxiliary metabolic potential of rural-associated phages enriched for carbon and amino acid energy metabolism. Conclusions: We report distinct differences in antimicrobial resistance gene profiles, enrichment of metabolic pathways and phage composition between rural and urban populations, as well as a higher burden of Clostridioides difficile disease in the urban population. Our results reveal that geography is the key driver of variation in urban and rural Indian microbiomes, with acute diarrheal disease, including C. difficile disease exerting a lesser impact. Future studies will be required to understand the potential role of dietary, cultural, and genetic factors in contributing to microbiome differences between rural and urban populations.en
dc.language.isoenen
dc.subjectClostridioides difficileen
dc.subjectGut Microbiomeen
dc.subjectIndiaen
dc.subjectAntimicrobial Resistanceen
dc.titleMetagenomics reveals impact of geography and acute diarrheal disease on the Central Indian human gut microbiome.en
dc.typeArticleen


This item appears in the following Collection(s)

Show simple item record